Research Interest: Basic experiment skills in molecular and cell biology, immunology and zoology as follows: Molecular biology:Regular skills of molecular biology;plasmid construction, including shRNA and overexpression, GFP fusion protein, nucleic acid extraction, purification. Biochemistry: protein expression analysis, including western blotting, immunoprecipitation and quantitative real-time RT-PCR; E.coli expressionsystem, soluble and insoluble protein purification. Cell biology: Mammalian cell culture; plasmid transfection; development of stable transfected cell lines; immunohistochemistry; immunocytochemistry. Others: Basic animal, especially mouse and rat handling skills;drosophila culture; familiar with ordinary operation of laser scanning confocal microscopy (Leica,Zeiss,Olympus), fluorescence microscopy, fluorescence spectrometer;familiar with ordinary statistic and bioinformatical softwares.
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