Research Interest: Bacteria of the genus Staphylococcus,
in particular the species S. aureus and virulence factors produced by this
species. In the so far carried out research, the results of which were
published in several peer-reviewed journals I analyzed:
1.The influence of
leucocytotoxic and superantygenic virulence factors produced by S. aureus
strains isolated from milk of cows with mastitis on the cytokine production and
gene expression in bovine leukocyte cultures in vitro;
2. the impact of staphylococcal exotoxins on the process of phagocytosis in
vitro;
3. the efficacy of treatment of mastitis by staphylococcal autovaccine;
4. the profile of resistance to methicillin of coagulase-negative staphylococci
isolated from animals;
5. the profile of the genes encoding superantigens in S. aureus and S. xylosus
isolates collected from cows;
6. the possibility of transfer of genes encoding superantigens between
staphylococci occurring in the same mammary gland;
7. genetic relatedness of staphylococci isolated within a herd of animals;
8. the biological activity of staphylococcal exosecretions against leukocytes
in vitro;
9. the impact of staphylococcal virulence factors on the morphology of
leukocytes in vitro.
I also analyzed and published in the GenBank several new 16S rRNA gene
sequences of bacteria representing different species of staphylococci,
identified by PCR-RFLP analysis of gap gene. Furthermore, as a contributor I
published in the GenBank the 16S rRNA gene sequences of bacterial isolates,
which were unidentified based on the polymorphism of gap gene, of which at
least two isolates represent new species or subspecies of the staphylococci.
The second direction of my research concerns the use of magnetic fields, in
particular the rotating magnetic field to modify the functional parameters and
pathogenicity potential of different species of microorganisms. At the present,
I’m analyzing the influence of magnetic field on metabolic processes in
microorganisms, both at the cellular and molecular level. As a continuation of
my first research mainstream microorganism used in these studies is primarily
S. aureus.
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