Davide Di Paola

Designation:
Researcher
Department:
Chemical, Biological, Pharmaceuticals and Environmental Sciences
University:
Messina
Country:
Italy
Email: Journal Associated: Annals of Environmental Science and Toxicology Biography:

March 1th, 2023 to date: Researcher in the Competition Sector 05 / C1- (SSD-BIO 07) ECOLOGY at the Department of

Chemical, Biological, Pharmaceuticals and Environmental Sciences of the

University of Messina.

• 05/2022 - 07/2022: Visiting Researcher at Marbio-UiT Artic University of Norway:

Extraction techniques of Bioactive Compounds from aquatic organism and

biotechnological applications

• 2019-2022: PhD in " Applied Biology and Experimental Medicine" (XXXV cycle)

at the Department of Chemical, Biological, Pharmaceuticals and Environmental

Sciences of the University of Messina.

• 2004-2014: Master Degree in CHEMISTRY AND PHARMACEUTICAL

TECHNOLOGY, awarded at the University of Messina.

SKILLS:

• Experience in screening complex mixtures (i.e. natural product extracts and

fractions) for biological activity in biochemical and cell-based assays

• Identification of known and potentially unknown bioactive compounds

(dereplication) using high-resolution tandem mass spectrometry

• Mass guided isolation of bioactive natural products from extracts and organisms,

purity determination.

• Structural elucidation of complex natural products using high resolution mass

spectrometry and spectroscopical tecniques such as NMR, IR and UV-Vis

spectroscopy.

• Isolation and cultivation of marine microorganisms (Bacteria and Fungi),

• Focus on samples of marine origin such as extracts from invertebrates, marine

fungi and bacteria,

• MOLECULAR BIOLOGY TECHNIQUES

• Use of microscopes, camera image acquisition and computer image processing,

preparation of samples and solutions in sterility, ability to work in a clean room

and under a laminar flow hood, cell cultures, determination of cell mortality by

MTT, electrophoresis on gel, western blot, nitrite and nitrate assay, comet assay,

immunofluorescence, immunohistochemistry, preparation and staining of

histological samples. ELISA , PCR.

 

Research Interest: Marine Ecology, Ecotoxicology, Marine Pharmacology

Indexing/Archiving